人乳酸脱氢酶(LDH)酶联免疫吸附检测试剂盒-开云kaiyun信誉（武汉）生物科技有限责任公司

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人乳酸脱氢酶(LDH)酶联免疫吸附检测试剂盒

ELK2236

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview 文献

Product name:	Human LDH(Lactate Dehydrogenase) ELISA Kit
Reactivity:	Human
Alternative Names:	Lactate Dehydrogenase
Assay Type:	Sandwich
Sensitivity:	0.057 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;Metabolic pathway;Tumor immunity;Infection immunity;Cardiovascular biology;Hepatology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LDH in the samples is then determined by comparing the OD of the samples to the standard curve.

日期：2023-01-05 IF：7.033

标准曲线

Concentration (ng/mL)	OD	Corrected OD
10.00	2.201	2.115
5.00	1.593	1.507
2.50	1.113	1.027
1.25	0.847	0.761
0.63	0.487	0.401
0.32	0.339	0.253
0.16	0.208	0.122
0.00	0.086	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant LDH and the recovery rates were calculated by comparing the measured value to the expected amount of LDH in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	80-93%	86%
Heparin plasma(n=5)	95-107%	101%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LDH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.