大鼠肿瘤坏死因子α(TNFa)酶联免疫吸附检测试剂盒-开云kaiyun信誉（武汉）生物科技有限责任公司

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大鼠肿瘤坏死因子α(TNFa)酶联免疫吸附检测试剂盒

ELK1396

规格：	价格：
48T	￥1680.00
96T	￥2400.00

下载说明 ①

Overview 文献

Product name:	Rat TNFa(Tumor Necrosis Factor Alpha) ELISA Kit
Reactivity:	Rat
Alternative Names:	TNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
Assay Type:	Sandwich
Sensitivity:	6.1 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Infection immunity;
Uniprot ID:	P16599
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNFa in the samples is then determined by comparing the OD of the samples to the standard curve.

日期：2021-10-20 IF：4.8
日期：2021-10-20 IF：4.06
日期：2019-06-06 IF：4.696
日期：2021-10-20 IF：3.211
日期：2021-10-20 IF：4.784
日期：2021-10-06 IF：3.503
日期：2021-12-28 IF：4.379
日期：2020-10-11 IF：4.077
日期：2021-08-13 IF：5.682
日期：2021-07-08 IF：5.81
日期：2021-10-18 IF：6.543
日期：2021-07-07 IF：2.447
日期：2020-02-20 IF：6.543
日期：2022-03-18 IF：5.194
日期：2020-04-24 IF：5.037
日期：2021-11-02 IF：8.322
日期：2022-04-28 IF：-
日期：2022-04-28 IF：-
日期：2022-07-08 IF：3.715
日期：2022-07-04 IF：2.65
日期：2022-07-05 IF：6.59
日期：2022-07-22 IF：1.564
日期：2022-07-05 IF：8.786
日期：2022-07-22 IF：1.564
日期：2022-08-17 IF：4.996
日期：2022-05-20 IF：-
日期：2022-02-15 IF：19.227
日期：2021-01-06 IF：-
日期：2022-10-03 IF：3.712
日期：2022-10-25 IF：-
日期：2022-12-15 IF：4.996
日期：2022-11-29 IF：4.631
日期：2022-12-13 IF：-
日期：2022-12-19 IF：4.253
日期：2022-02-28 IF：6.819
日期：2023-01-17 IF：15.863
日期：2023-04-10 IF：5.8
日期：2023-04-12 IF：4.6

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.345	2.256
500.00	1.769	1.680
250.00	1.225	1.136
125.00	0.917	0.828
62.50	0.521	0.432
31.25	0.366	0.277
15.63	0.207	0.118
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TNFa and the recovery rates were calculated by comparing the measured value to the expected amount of TNFa in samples.

Matrix	Recovery range	Average
serum(n=5)	80-90%	85%
EDTA plasma(n=5)	84-106%	100%
Heparin plasma(n=5)	87-93%	90%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFa and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.