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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 2.136 | 2.048 |
500.00 | 1.629 | 1.541 |
250.00 | 1.182 | 1.094 |
125.00 | 0.933 | 0.845 |
62.50 | 0.589 | 0.501 |
31.25 | 0.357 | 0.269 |
15.63 | 0.234 | 0.146 |
0.00 | 0.088 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 87-99% | 93% |
EDTA plasma(n=5) | 85-97% | 91% |
Heparin plasma(n=5) | 90-103% | 96% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 87-94% | 91-104% | 95-107% | 82-96% |
EDTA plasma(n=5) | 81-94% | 93-107% | 93-102% | 91-105% |
Heparin plasma(n=5) | 89-98% | 81-94% | 96-105% | 92-103% |
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