雌二醇(E2)酶联免疫吸附检测试剂盒-开云kaiyun信誉（武汉）生物科技有限责任公司

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雌二醇(E2)酶联免疫吸附检测试剂盒

ELK1208

规格：	价格：
48T	￥1680.00
96T	￥2400.00

下载说明 ①

Overview 文献

Product name:	E2(Estradiol) ELISA Kit
Reactivity:	General
Alternative Names:	17B-Estradiol; Oestradiol; Beta-Estradiol
Assay Type:	Competitive Inhibition
Sensitivity:	4.45 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	2h
Research Area:	Endocrinology;Reproductive science;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with E2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

日期：2022-05-30 IF：6.208
日期：2022-12-29 IF：2.923
日期：2024-01-18 IF：5.4

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	0.189
500.00	0.397
250.00	0.596
125.00	0.833
62.50	1.197
31.25	1.592
15.63	1.789
0.00	2.123

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant E2 and the recovery rates were calculated by comparing the measured value to the expected amount of E2 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-95%	89%
EDTA plasma(n=5)	88-102%	95%
Heparin plasma(n=5)	79-97%	88%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.