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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
500.00 | 2.328 | 2.260 |
250.00 | 2.045 | 1.977 |
125.00 | 1.412 | 1.344 |
62.50 | 0.780 | 0.712 |
31.25 | 0.444 | 0.376 |
15.63 | 0.262 | 0.194 |
7.82 | 0.132 | 0.064 |
0.00 | 0.068 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 97-105% | 101% |
EDTA plasma(n=5) | 87-99% | 92% |
Heparin plasma(n=5) | 88-107% | 94% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 86-93% | 91-103% | 87-98% | 92-99% |
EDTA plasma(n=5) | 89-99% | 83-96% | 98-104% | 88-101% |
Heparin plasma(n=5) | 85-102% | 84-99% | 97-101% | 89-102% |
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