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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
10.00 | 2.126 | 2.057 |
5.00 | 1.685 | 1.616 |
2.50 | 1.385 | 1.316 |
1.25 | 0.866 | 0.797 |
0.63 | 0.596 | 0.527 |
0.32 | 0.366 | 0.297 |
0.16 | 0.138 | 0.069 |
0.00 | 0.069 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 80-98% | 89% |
EDTA plasma(n=5) | 82-94% | 88% |
Heparin plasma(n=5) | 84-98% | 91% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 79-92% | 88-96% | 77-91% | 85-93% |
EDTA plasma(n=5) | 87-102% | 96-105% | 85-93% | 89-97% |
Heparin plasma(n=5) | 95-104% | 87-96% | 88-101% | 82-95% |
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