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Overview
标准曲线
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 1000.00 | 2.266 | 2.083 |
| 500.00 | 1.803 | 1.620 |
| 250.00 | 1.386 | 1.203 |
| 125.00 | 0.971 | 0.788 |
| 62.50 | 0.630 | 0.447 |
| 31.25 | 0.414 | 0.231 |
| 15.63 | 0.252 | 0.069 |
| 0.00 | 0.183 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant EG-VEGF and the recovery rates were calculated by comparing the measured value to the expected amount of EG-VEGF in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 82-95% | 88% |
| EDTA plasma(n=5) | 85-97% | 91% |
| Heparin plasma(n=5) | 79-93% | 86% |
线性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EG-VEGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 95-104% | 97-106% | 85-98% | 81-92% |
| EDTA plasma(n=5) | 83-92% | 96-102% | 81-93% | 79-91% |
| Heparin plasma(n=5) | 89-101% | 85-96% | 95-104% | 82-90% |
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