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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
2000.00 | 1.842 | 1.777 |
1000.00 | 1.330 | 1.265 |
500.00 | 0.875 | 0.810 |
250.00 | 0.484 | 0.419 |
125.00 | 0.395 | 0.330 |
62.50 | 0.239 | 0.174 |
31.25 | 0.162 | 0.097 |
0.00 | 0.065 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 84-93% | 89% |
EDTA plasma(n=5) | 81-103% | 87% |
Heparin plasma(n=5) | 93-105% | 99% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 88-96% | 90-101% | 93-102% | 85-94% |
EDTA plasma(n=5) | 92-103% | 81-92% | 78-87% | 91-99% |
Heparin plasma(n=5) | 92-104% | 93-99% | 87-953% | 82-93% |
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