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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
500.00 | 1.994 | 1.911 |
250.00 | 1.577 | 1.494 |
125.00 | 1.116 | 1.033 |
62.50 | 0.841 | 0.758 |
31.25 | 0.517 | 0.434 |
15.63 | 0.340 | 0.257 |
7.82 | 0.236 | 0.153 |
0.00 | 0.083 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 87-95% | 91% |
EDTA plasma(n=5) | 78-92% | 85% |
Heparin plasma(n=5) | 92-106% | 99% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 88-102% | 87-102% | 85-96% | 87-101% |
EDTA plasma(n=5) | 87-101% | 87-96% | 82-93% | 89-97% |
Heparin plasma(n=5) | 85-93% | 98-105% | 87-98% | 95-102% |
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